Systems Proteomics & Adaptive Mass Spectrometry

University of Washington

Department of Genome Sciences

Quantitative Proteomics

Extending technologies to improve the sensitivity and efficiency of quantitative proteomics methods. Multiplexed proteomics (analysis of multiple samples within a single LC-MS/MS run) enable high-throughput methodologies for precise analysis of dynamic signaling networks. These methods can be readily employed across a range of systems, from bacteria and single celled organisms through to human cell lines and primary tissue.

Real-time, Adaptive Instrument Control

We have several projects dedicated the development and use of adaptive, real-time instrument control. This includes extending the capabilities of the real-time search (RTS) platform for multiplexed proteomics. RTS enables a two-fold increase in acquisition time for multiplexed quantitative proteomics over canonical methods without sacrificing quantitative accuracy.